Journal: Animals : an Open Access Journal from MDPI

Article Title: Multi-Omics Insights into Postnatal Skeletal Muscle Development in Duroc Pigs

doi: 10.3390/ani15182715

Figure Lengend Snippet: Phenotypic assessment of SOL, GAS, and PMM in Duroc pigs. ( A ) Experimental design diagram. ( B ) Phenotypic diagrams of SOL, GAS, LDM, and PMM are presented, with LDM as the control for PMM ( n = 6). ( C ) HE staining of SOL, GAS, and PMM across three developmental stages ( n = 6); Scale bar, 50 μm. ( D ) At 120 days of age, MYH7 antibody immunostaining was performed on SOL, GAS, and PMM ( n = 3). ( E , F ) At 120 days of age, the mRNA expression levels of MYH7 and MYH4 in SOL, GAS, and PMM were detected using qRT-PCR ( n = 3). ( G ) Protein expression levels of MYH7 and MYH1 during the development of the SOL, GAS, and PMM were analyzed by Western Blot ( n = 3).

Article Snippet: Western blot analysis was performed with the following primary antibodies: MYH7 (1:1000, 22280-1-AP, Proteintech Group, Inc., Wuhan, China), MYH1 (1:10000, 67299-1-Ig, Proteintech Group, Inc., Wuhan, China), and Anti-GAPDH (1:10000, 10494-1-AP, Proteintech Group, Inc., Wuhan, China).

Techniques: Control, Staining, Immunostaining, Expressing, Quantitative RT-PCR, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: The long noncoding RNA lnc-H19 is important for endurance exercise by maintaining slow muscle fiber types

doi: 10.1016/j.jbc.2023.105281

Figure Lengend Snippet: Lnc-H19 promotes the conversion of fast muscle fibers to slow muscle fibers in C2C12 cell. A , the mRNA expression level of lnc-H19 was interfered (n = 3 biological replicates). B , immunofluorescence staining of fast-MyHC after interference of lnc-H19 (scale bar represents 200 μm). Each group of three biological repeats. C and J , B and I , statistics of immunofluorescence intensity. Five views per photo were selected and counted, and the average was set as the value of this sample statistical analysis (n = 3 biological replicates). D and K , MyHC isoform composition (%) (n = 3 biological replicates). E and L , the protein expression levels of MyHCI, MyHC IIA, MyHC IIX, and MyHC IIB (n = 3 biological replicates). F and M, quantification of MyHC I, MyHC IIA, MyHC IIX, MyHC IIB, and PGC-1α protein (n = 3 biological replicates). G and N , the expression of mitochondrial-related genes (n = 3 biological replicates). H , the mRNA expression level of lnc-H19 was overexpressed (n = 3 biological replicates). I , immunofluorescence staining of fast-MyHC after overexpression of lnc-H19 (scale bar represents 200 μm). Each group of three biological repeats. The results are expressed as mean ± SD, ∗ p < 0.05 and ∗∗ p < 0.01. lnc, long noncoding.

Article Snippet: The primary antibodies targeted the following proteins: MyHC I (1:1000 dilution; DSHB), MyHC IIA (1:1000 dilution; DSHB), MyHC IIX (1:1000 dilution; DSHB), MyHC IIB (1:1000 dilution; DSHB), Fast-MyHC (1:1000 dilution; Sigma), Slow-MyHC (1:1000 dilution; Sigma), and β-tubulin (1:200 dilution; Santa Cruz Biotechnology).

Techniques: Expressing, Immunofluorescence, Staining, Over Expression

Journal: The Journal of Biological Chemistry

Article Title: The long noncoding RNA lnc-H19 is important for endurance exercise by maintaining slow muscle fiber types

doi: 10.1016/j.jbc.2023.105281

Figure Lengend Snippet: Primer sequence

Article Snippet: The primary antibodies targeted the following proteins: MyHC I (1:1000 dilution; DSHB), MyHC IIA (1:1000 dilution; DSHB), MyHC IIX (1:1000 dilution; DSHB), MyHC IIB (1:1000 dilution; DSHB), Fast-MyHC (1:1000 dilution; Sigma), Slow-MyHC (1:1000 dilution; Sigma), and β-tubulin (1:200 dilution; Santa Cruz Biotechnology).

Techniques:

Journal: eLife

Article Title: Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency

doi: 10.7554/eLife.15461

Figure Lengend Snippet: Primary antibodies used for immunofluorescence. The monoclonal antibodies for Pax7, Myog, Myosin, IIx type myosin, IIa type myosin, non-IIx type myosin, I type myosin, and embryonic myosin, developed by Stefano Schiaffino, C Lucas, A Kawakami, WE Wright, DA Fischman, and HM Blau were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. DOI: http://dx.doi.org/10.7554/eLife.15461.014

Article Snippet: Anti-myosin (IIX) , mouse (IgM) , 1:2 , DSHB 6H1 (RRID: AB_2314830 ).

Techniques: Immunofluorescence, Bioprocessing

Journal: Science Advances

Article Title: Lmod2 is necessary for effective skeletal muscle contraction

doi: 10.1126/sciadv.adk1890

Figure Lengend Snippet: Lmod2 WT + cGFP and KO + cGFP-Lmod2 skeletal muscle cross sections were stained with antibodies against different MHC isoforms. Representative images of EDL ( A ) or soleus ( B ) MHC fiber type staining. Top: MHC type I (blue), MHC type IIB (green), and laminin (red). Middle: MHC type IIA (green) and laminin (red). Bottom: MHC type IIX (blue) and laminin (red). Quantification of the number of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( C ) or soleus ( E ) cross-sections shown as percent of total fibers. Minimum Feret diameter of MHC type I–, IIA–, IIB–, or IIX–positive fibers in EDL ( D ) or soleus ( F ) cross sections. Scale bars, 100 μm. All values are means ± SD; P < 0.05 was considered significant, unpaired t test; * P < 0.05, ** P < 0.01; n = 4 mice per genotype.

Article Snippet: The following primary antibodies were used: MHC I [3.5 mg/ml; BA-F8 IgG2b, Developmental Studies Hybridoma Bank (DSHB)], MHC IIB (9 mg/ml; BF-F3 IgM, DSHB), MHC IIA (3.5 mg/ml; SC-71 IgG1, DSHB), MHC IIX exclusion (3.5 mg/ml; BF-35 IgG1, DSHB), pan-myosin (9 mg/ml; A4-1025 IgG2a, DSHB), and rabbit polyclonal laminin (1:400; L9393, Sigma-Aldrich).

Techniques: Staining